Madikizela-Mandela was Mandela’s second wife. They divorced in 1996 after 38 years of marriage.
Madikizela-Mandela reportedly wanted a R20 million divorce settlement, which she said was half of Mandela’s assets.
Her claim was unsuccessful.
The anti-apartheid veteran was left out of the will that was read by executors in Johannesburg on Monday.
Her children and grandchildren were the beneficiaries.
Mandela left R3m each for their two children Zenani Mandela-Dlamini and Zindzi Mandela.
Zenani’s children: Zinhle, Zaziwe and Zamaswazi would receive R100,000 each.
Bambatha, Zondwa, Zwelabo and Zoleka, who are Zindzi’s children, would also receive R100,000 each.
Mandela’s great grandchild, Zozuko Mandela, who is Zamaswazi’s child, will get R100,000 from the estate.
The provisional assessment of Mandela’s estate was about R46 million, Deputy Chief Justice Dikgang Moseneke told reporters as he released Mandela’s will.